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c9 1 200 novus biologicals nbp2 15952  (Novus Biologicals)


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    Novus Biologicals c9 1 200 novus biologicals nbp2 15952
    C9 1 200 Novus Biologicals Nbp2 15952, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/novus+biologicals+nbp2/Complement+Component+C9+Antibody/pmc13128952-289-40-42
    Average 94 stars, based on 2 article reviews
    c9 1 200 novus biologicals nbp2 15952 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Defining the cell and molecular origins of the primate ovarian reserve
    Article Snippet: .. Primary Antibody Concentration Source Catalogue no. / Identifier anti-AMH* 1:100 ABD Serotec MCA2246T RRID:AB_2226471 anti-AP2g (TFAP2C) 1:200 Santa Cruz sc-12762 RRID:AB_667770 anti-cleaved Caspase3 1:200 Cell Signaling 9661 RRID:AB_2341188 anti-CK18 1:200 Abcam ab7797 RRID:AB_306086 anti-CK19 1:200 Novus Biologicals NBP2-15186 RRID: AB_2737392 anti-cKIT 1:200 DAKO A4502 anti-cleaved PARP 1:200 Cell Signaling 5625 RRID:AB_10699459 anti-CYP17A1 1:200 Santa Cruz SC-374244 RRID:AB_10988393 anti-CYP19A1 1:200 Novus Biologicals NB100-1596 RRID:AB_10000919 anti-Decorin 1:200 R&D Systems AF143 RRID:AB_354790 anti-GATA2 1:100 Abcam ab109241 RRID: AB_10865130 anti-GATA4* 1:200 Abcam ab84593 RRID:AB_10670538 anti-GATM 1:200 Abcam ab119269 RRID:AB_10902241 anti-FOXL2 1:100 Novus Biologicals NB100-1277 RRID:AB_2106188 anti-Ki67 (mouse) 1:200 BD Biosciences 556003 RRID: AB_396287 anti-Ki67 (rat) 1:200 Invitrogen 14-5698-80 RRID:AB_10853185 anti-Laminin 1:100 Abcam ab11575 RRID: AB_298179 anti-NR2F2 1:200 R&D Systems PP-H7147-00 RRID:AB_2155627 anti-NR5A1 1:200 R&D Systems PP-N1665-00 RRID:AB_2251509 anti-PAPP-A 1:100 R&D Systems AF2487SP RRID:AB_2159346 anti-PAX8 1:100 Novus Biologicals NBP2-29903 anti-PRDM1 1:100 Cell Signaling 9115S RRID:AB_2169699 anti-SOX17 1:500 R&D Systems AF1924 RRID: AB_355060 anti-VASA (rabbit) 1:200 Abcam ab13840 RRID: AB_443012 anti-VASA (goat) 1:200 R&D Systems AF2030 RRID: AB_2277369 anti-WT1 1:100 Abcam ab89901 RRID:AB_2043201 *Antigen retrieval with sodium citrate buffer Supplementary Table 4. ..

    Article Title: Coagulation factor XII haploinsufficiency is protective against venous thromboembolism in a population-scale multidimensional analysis
    Article Snippet: .. The primary antibody used was clone 10-11-5 (Novus Biologicals NBP2-41375). (D) FXIIa activity of plasma from MGH Biobank participants with wildtype F12 (WT, N=6) or ESS1 (N=6), shown as aggregate kinetic curves (left) and individual values reflecting plasma FXIIa concentration (right, mean ± SD shown, significance determined by unpaired two-tailed t-test). ..

    other:

    Article Title: Ataxia and cerebellar hypoexcitability in a mouse model of SCN1B -linked Dravet syndrome
    Article Snippet: Quantitative PCR was performed using SYBR Green (Applied Biosystems) and gene-specific primers ( Scn1a , Scn2a , Scn3a , Scn4a , Scn8a , Scn9a , Scn4b , Actb ; Integrated DNA Technologies) on a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems).

    Article Title: Splice-switching antisense oligonucleotides correct cryptic exon inclusion and restore SDCCAG8 protein in Bardet-Biedl Syndrome
    Article Snippet: Wet transfer was done using a nitrocellulose membrane in NuPAGE transfer buffer (Invitrogen NP00061).

    Article Title: Extensible Immunofluorescence (ExIF) accessibly generates high-plexity datasets by integrating standard 4-plex imaging data
    Article Snippet: CD44 Mouse 1:500 Cell Signaling Technology 3570S Indirect IF CD44std Mouse 1:500 Thermo-Fischer Scientific BMS113 Indirect IF CD44v9 Mouse 1:500 Novus Biologicals NBP2-53204 Indirect IF, 4i COX IV Mouse 1:500 Abcam ab33985 Indirect IF, 4i E-Cadherin Mouse 1:500 BD Biosciences 610181 Indirect IF EpCAM Mouse 1:500 Cell Signaling Technology 2929S Indirect IF Fibrillarin Rabbit 1:500 Abcam ab5821 Indirect IF, 4i GM130 Mouse 1:500 BD Biosciences 610823 Indirect IF, 4i N-Cadherin Mouse 1:200 Cell Signaling Technology 14215S Indirect IF NF-kB p65 Rabbit 1:500 Cell Signaling Technology 8242S Indirect IF, 4i PTEN Mouse 1:500 Thermo-Fischer Scientific 32-5800 Indirect IF Vimentin Mouse 1:500 Abcam ab8978 Indirect IF α-Tubulin Mouse 1:500 Abcam ab7291 Indirect IF β-catenin Rabbit 1:500 Thermo-Fischer Scientific 71-2700 Indirect IF, 4i Fluorescent Labels 4’, 6-diamidino-2phenylindole (DAPI) N/A 1:2000 (Indirect IF, 4i), 1:4000 (CycIF) Sigma-Aldrich Pty Ltd D9532 Indirect IF, 4i, CycIF Atto-647 Phalloidin N/A 1:2000 Atto-Tec GmbH AD647-81 Indirect IF, 4i,

    Protein Concentration:

    Article Title: MUC13-Associated Molecular Interactome in Pancreatic Cancer
    Article Snippet: .. To quantify the protein concentration of MUC13 in various PDAC cell lines, enzyme-linked immunosorbent assay (ELISA) was carried out using a commercial kit from Novus Biologicals (catalog no. NBP2-76698) following the manufacturer’s protocol. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: MUC13-Associated Molecular Interactome in Pancreatic Cancer
    Article Snippet: .. To quantify the protein concentration of MUC13 in various PDAC cell lines, enzyme-linked immunosorbent assay (ELISA) was carried out using a commercial kit from Novus Biologicals (catalog no. NBP2-76698) following the manufacturer’s protocol. ..

    Western Blot:

    Article Title: Invadopodia-Mediated Remodeling of the Lymphatic Endothelium Drives Cancer Cell Lymphatic Dissemination and is Regulated by a CCR7-Vav2-Rac3 Signaling axis
    Article Snippet: All reagents were purchased from either Sigma-Aldrich (St. Louis, MO) or Thermo Fisher Scientific (Nepean, ON, Canada) unless otherwise stated. .. Antibodies specific for following proteins were purchased from the suppliers indicated: Tks5 (for western blot: Cell Signaling 16619S; 1:250), Tks5 (for Immunofluorescence: Millipore Sigma 09-403), β-actin (for western blot: Sigma A5441; 1:5000), ZO-1 (Thermo Fisher Scientific 33-9100; 1:50), Vinculin (for western blot: Abcam, ab91495, 1:3000), VE-Cadherin (Abcam ab33168; 1:50), CCR7 (for Immunofluorescence (1:500) and Immunoprecipitation (1ug): Novus Biologicals NBP2-67324), CCR7 (for western blot: Abcam ab 191575, 1:500), Vav2 (Proteintech 21924-1-AP,1:500), Rac3 (Invitrogen MA5-53241,1:500), Phosphotyrosine (for western blot: Invitrogen 14-5001-82, 1:1500) and Rabbit Isotype control (Biolegend, Cat. No. 910801). .. The secondary antibodies (1:3000) and phalloidin labeled with Alexa Fluor 647 (1: 1000) were purchased from Invitrogen (Burlington, Canada).

    Immunofluorescence:

    Article Title: Invadopodia-Mediated Remodeling of the Lymphatic Endothelium Drives Cancer Cell Lymphatic Dissemination and is Regulated by a CCR7-Vav2-Rac3 Signaling axis
    Article Snippet: All reagents were purchased from either Sigma-Aldrich (St. Louis, MO) or Thermo Fisher Scientific (Nepean, ON, Canada) unless otherwise stated. .. Antibodies specific for following proteins were purchased from the suppliers indicated: Tks5 (for western blot: Cell Signaling 16619S; 1:250), Tks5 (for Immunofluorescence: Millipore Sigma 09-403), β-actin (for western blot: Sigma A5441; 1:5000), ZO-1 (Thermo Fisher Scientific 33-9100; 1:50), Vinculin (for western blot: Abcam, ab91495, 1:3000), VE-Cadherin (Abcam ab33168; 1:50), CCR7 (for Immunofluorescence (1:500) and Immunoprecipitation (1ug): Novus Biologicals NBP2-67324), CCR7 (for western blot: Abcam ab 191575, 1:500), Vav2 (Proteintech 21924-1-AP,1:500), Rac3 (Invitrogen MA5-53241,1:500), Phosphotyrosine (for western blot: Invitrogen 14-5001-82, 1:1500) and Rabbit Isotype control (Biolegend, Cat. No. 910801). .. The secondary antibodies (1:3000) and phalloidin labeled with Alexa Fluor 647 (1: 1000) were purchased from Invitrogen (Burlington, Canada).

    Immunoprecipitation:

    Article Title: Invadopodia-Mediated Remodeling of the Lymphatic Endothelium Drives Cancer Cell Lymphatic Dissemination and is Regulated by a CCR7-Vav2-Rac3 Signaling axis
    Article Snippet: All reagents were purchased from either Sigma-Aldrich (St. Louis, MO) or Thermo Fisher Scientific (Nepean, ON, Canada) unless otherwise stated. .. Antibodies specific for following proteins were purchased from the suppliers indicated: Tks5 (for western blot: Cell Signaling 16619S; 1:250), Tks5 (for Immunofluorescence: Millipore Sigma 09-403), β-actin (for western blot: Sigma A5441; 1:5000), ZO-1 (Thermo Fisher Scientific 33-9100; 1:50), Vinculin (for western blot: Abcam, ab91495, 1:3000), VE-Cadherin (Abcam ab33168; 1:50), CCR7 (for Immunofluorescence (1:500) and Immunoprecipitation (1ug): Novus Biologicals NBP2-67324), CCR7 (for western blot: Abcam ab 191575, 1:500), Vav2 (Proteintech 21924-1-AP,1:500), Rac3 (Invitrogen MA5-53241,1:500), Phosphotyrosine (for western blot: Invitrogen 14-5001-82, 1:1500) and Rabbit Isotype control (Biolegend, Cat. No. 910801). .. The secondary antibodies (1:3000) and phalloidin labeled with Alexa Fluor 647 (1: 1000) were purchased from Invitrogen (Burlington, Canada).

    Control:

    Article Title: Invadopodia-Mediated Remodeling of the Lymphatic Endothelium Drives Cancer Cell Lymphatic Dissemination and is Regulated by a CCR7-Vav2-Rac3 Signaling axis
    Article Snippet: All reagents were purchased from either Sigma-Aldrich (St. Louis, MO) or Thermo Fisher Scientific (Nepean, ON, Canada) unless otherwise stated. .. Antibodies specific for following proteins were purchased from the suppliers indicated: Tks5 (for western blot: Cell Signaling 16619S; 1:250), Tks5 (for Immunofluorescence: Millipore Sigma 09-403), β-actin (for western blot: Sigma A5441; 1:5000), ZO-1 (Thermo Fisher Scientific 33-9100; 1:50), Vinculin (for western blot: Abcam, ab91495, 1:3000), VE-Cadherin (Abcam ab33168; 1:50), CCR7 (for Immunofluorescence (1:500) and Immunoprecipitation (1ug): Novus Biologicals NBP2-67324), CCR7 (for western blot: Abcam ab 191575, 1:500), Vav2 (Proteintech 21924-1-AP,1:500), Rac3 (Invitrogen MA5-53241,1:500), Phosphotyrosine (for western blot: Invitrogen 14-5001-82, 1:1500) and Rabbit Isotype control (Biolegend, Cat. No. 910801). .. The secondary antibodies (1:3000) and phalloidin labeled with Alexa Fluor 647 (1: 1000) were purchased from Invitrogen (Burlington, Canada).

    Staining:

    Article Title: Ataxia and cerebellar hypoexcitability in a mouse model of SCN1B-linked Dravet syndrome.
    Article Snippet: cDNA was generated from 1–1.5 mg of RNA using Reverse Transcriptase SuperScript III (RT SS III), random primers (Invitrogen), and deoxynucleotide triphosphates (dNTPs) (Invitrogen). .. To study Scn8a levels in PCs and MLIs, sections were stained with Mm-Scn8a-C1 (ACD Biosystems 434191) + Mm-Pvalb-C3 (ACD Biosystems 421931) followed by IHC with anti-calbindin 1:1,000 (CK, Novus Biologicals NBP2-50028). .. Salt buffers, 0.1 M DTT, RNase Out (Invitrogen), and RT SS III were added, and reactions were incubated at 25°C for 5 minutes, 50°C for 60 minutes, and 70°C for 15 minutes.

    Immunohistochemistry:

    Article Title: Ataxia and cerebellar hypoexcitability in a mouse model of SCN1B-linked Dravet syndrome.
    Article Snippet: cDNA was generated from 1–1.5 mg of RNA using Reverse Transcriptase SuperScript III (RT SS III), random primers (Invitrogen), and deoxynucleotide triphosphates (dNTPs) (Invitrogen). .. To study Scn8a levels in PCs and MLIs, sections were stained with Mm-Scn8a-C1 (ACD Biosystems 434191) + Mm-Pvalb-C3 (ACD Biosystems 421931) followed by IHC with anti-calbindin 1:1,000 (CK, Novus Biologicals NBP2-50028). .. Salt buffers, 0.1 M DTT, RNase Out (Invitrogen), and RT SS III were added, and reactions were incubated at 25°C for 5 minutes, 50°C for 60 minutes, and 70°C for 15 minutes.

    Activity Assay:

    Article Title: Coagulation factor XII haploinsufficiency is protective against venous thromboembolism in a population-scale multidimensional analysis
    Article Snippet: .. The primary antibody used was clone 10-11-5 (Novus Biologicals NBP2-41375). (D) FXIIa activity of plasma from MGH Biobank participants with wildtype F12 (WT, N=6) or ESS1 (N=6), shown as aggregate kinetic curves (left) and individual values reflecting plasma FXIIa concentration (right, mean ± SD shown, significance determined by unpaired two-tailed t-test). ..

    Clinical Proteomics:

    Article Title: Coagulation factor XII haploinsufficiency is protective against venous thromboembolism in a population-scale multidimensional analysis
    Article Snippet: .. The primary antibody used was clone 10-11-5 (Novus Biologicals NBP2-41375). (D) FXIIa activity of plasma from MGH Biobank participants with wildtype F12 (WT, N=6) or ESS1 (N=6), shown as aggregate kinetic curves (left) and individual values reflecting plasma FXIIa concentration (right, mean ± SD shown, significance determined by unpaired two-tailed t-test). ..



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